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2 edition of Production of peptide growth factors by oncogene-transformed balb serum-free mouse embryo cells found in the catalog.

Production of peptide growth factors by oncogene-transformed balb serum-free mouse embryo cells

Yung-jin Chang

Production of peptide growth factors by oncogene-transformed balb serum-free mouse embryo cells

by Yung-jin Chang

  • 251 Want to read
  • 28 Currently reading

Published .
Written in English

    Subjects:
  • Cell transformation.,
  • Growth factors.,
  • Oncogenes.

  • Edition Notes

    Statementby Yung-Jin Chang.
    The Physical Object
    Pagination41 leaves, bound :
    Number of Pages41
    ID Numbers
    Open LibraryOL15528960M

    Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media. Zhan X, Goldfarb M. We report conditions for the efficient growth of NIH 3T3 and BALB/c 3T3 cells cultured in a defined medium supplemented with either platelet-derived growth factor (PDGF) or pituitary-derived fibroblast growth factor (FGF).Cited by: Nov 01,  · A serum-free clonal density growth assay was developed for the quantification of the biological activity of human recombinant insulin-like growth factor I (IGF-I). The assay measures IGF-I stimulated growth of Balb/c 3T3 cells cultured over 4 d on poly-d-lysine-coated plastic surfaces in a serum-free medium formulation composed of a 1∶1 (vol/vol) mixture of Ham's F12 and Dulbecco's Cited by:

    Start studying Biology Chapter 11 Vocab. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Search. results in the uncontrolled production of white blood cells. growth factors. Start studying A&P Chapter Learn vocabulary, terms, and more with flashcards, games, and other study tools.

    Cellular Assays of Oncogene Transformation I. INTRODUCTION A critical step in the validation of the role of candidate oncogenes in oncogenesis involves a demonstration of their ability to endow nonneoplastic cells with growth properties characteristics of tumor cells. Jan 29,  · Introduction: Introduction Naturally occurring substance capable of stimulating cellular growth, proliferation and cellular differentiation, cell locomotion, contratility and angiogenesis Protein or a steroid hormone Act as signaling molecules between cells Eg: cytokines and hormones They often promote cell differentiation and maturation, which varies between growth factors. eg: bone.


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Production of peptide growth factors by oncogene-transformed balb serum-free mouse embryo cells by Yung-jin Chang Download PDF EPUB FB2

The presence of myc oncogene, when cotransformed with ras oncogene, increased the rapid growth ability of the ras oncogene-transformed cells in the minus EGF serum-free culture, developing earlier tumor formation in mice and producing more TGF-alpha into the conditioned akikopavolka.com by: 1.

Production of peptide growth factors by oncogene-transformed balb serum-free mouse embryo cells. Abstract. Graduation date: DNA from the human squamous carcinoma cell line, SK-MES-1, and the\ud plasmids, pUC EJ containing H-ras oncogene, pSVc-mycl containing\ud cellular myc gene, pNRSac containing N-ras oncogene, pBR SV containing.

We report conditions for the efficient growth of NIH 3T3 and BALB/c 3T3 cells cultured in a defined medium supplemented with either platelet-derived growth factor (PDGF) or pituitary-derived fibroblast growth factor (FGF).Cited by: Peptide growth factors, their associated proteins (carrier proteins and membrane receptors), and proto-oncogenes (the normal, cellular counterparts of retroviral oncogenic proteins) are intimately involved in normal and abnormal cellular growth regulation.

Jun 01,  · Serum-free mouse embryo (SFME) cells are a cell line derived in medium in which serum is replaced with growth factors and other supplements.

These cells display unusual properties: a) they do not lose proliferative potential or show gross chromosomal aberration upon extended culture, b) they depend on epidermal growth factor (EGF) for survival, and c) they are reversibly growth inhibited by plasma and akikopavolka.com by: 3.

Peptide growth factors and preimplantation. The problem of the mouse embryo as a model. stimulating factor control production of blood cells such as. Jan 01,  · To test the biological activity of the purified hEb-peptide, × 10 5 cells were suspended and washed with a serum free medium, and seeded into a well plate.

Different doses of the recombinant hEb-peptide that has been purified, acetone precipitated, and lyophilized were reconstituted in PBS and added into each well at a final concentration range of – akikopavolka.com: Jay H. Lo, Thomas T. Chen. Transforming growth factor beta 1 (1 ng/ml) caused death of serum-free mouse embryo cells cultured in a medium consisting of a mixture of Dulbecco's Modified Eagle's medium and Ham's F Transforming growth factor??1 stimulates the production of basic fibroblast growth factor binding proteoglycans in Balb/c3T3 cells.

Cells-Mouse Balb/c3T3 cells were obtained from the American. Growth Factors: Mechanism of Action and Relation to Oncogenes 19 Reddy, E. P., Smith, M. J., and Srinivasan, A. Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other one gene products with tyrosine-specific kinase akikopavolka.com by: Mar 11,  · Interestingly, HEK cells may be particularly attractive because early studies demonstrated that 10% horse serum can effectively promote cell growth in suspension culture.

58 Nevertheless, despite these alternatives FBS is almost exclusively used during over-expression experiments since high cellular viability and growth rate are prerequisites for obtaining high protein akikopavolka.com by: Assoian RK, Grotendorst GR, Miller DM, Sporn MB.

Cellular transformation by coordinated action of three peptide growth factors from human platelets. Nature. ()– Twardzik DR, Sherwin SA, Ranchalis J, Todaro GJ. Transforming growth factors in the urine of normal, pregnant, and tumor-bearing akikopavolka.com by: The 28H6 gene is a serum- and polypeptide growth factor-inducible delayed-early response gene encoding an ~ kb mRNA that is expressed at maximal levels ~ 12 h after mitogen additionIt encodes a amino-acid protein with a significant degree of sequence identity to members of the prolactin–growth hormone family (43).Cited by: Production of Peptide Growth Factors by Oncogene-Transformed Balb Serum-free Mouse Embryo Cells, Thesis.

Protease nexin-1, an antithrombin with neurite outgrowth activity, is reduced in Alzheimer disease. Both peptide growth factors and classical tropic hormones, are capable of rapidly modulating transcription through the induction of genes (fosljun) that encode nuclear transregulator proteins.

peptide growth factors / cell cycle Rum- Facteurs de croissance peptidiques et contre du cycle akikopavolka.com: HA Armelin. Abstract.

Effects of oncogenes such as c-myc,c-fos, v-myb, and c-Ha-ras were examined on the exogenous protein production by cultured animal akikopavolka.coml plasmids expressing oncogenes were constructed as effector plasmids and a plasmid containing the IL-6 gene regulated by the SRa promoter as a reporter akikopavolka.com by: 6.

Abstract. Activin, a peptide growth factor, is a member of the transforming growth factor-β (TGF-β) superfamily. It was originally isolated from follicle fluid as a gonadal hormone that stimulates follicle-stimulating hormone (FSH) secretion and is identical to EDF, the erythroid differentiation factor (which stimulates erythroleukemia cells to differentiate into hemoglobin-producing cells) ().Cited by: 6.

Production of Peptide Growth Factors by Oncogene-Transformed Balb Serum-Free Mouse Embryo Cells INTRODUCTION General Mechanisms of Oncogene Action Growth factors are defined as polypeptides that stimulate cell proliferation through binding to ubiquitous cell membrane receptors with specific high-affinities.

Serum-free defined media have been developed for assay of the mitogenic effects of growth factors on human MCF-7, human TD, and mouse COMMA-D mammary cells as well as for identification of.

The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft akikopavolka.com by:.

May 01,  · Polypeptide growth factors produced by tumor cells and virus-transformed cells: a possible growth advantage for the producer cells. in: Hormones and Cell Culture, Book A. Cold Spring Harbor Conferences on Cell akikopavolka.com by: Jul 01,  · Accordingly, K-fgf-transformed NIH 3T3 cells grew in serum-free medium, consistent with an autocrine mechanism of growth.

We have also expressed the protein encoded in the K-fgf protooncogene in COS-1 cells, and it was indistinguishable in its molecular weight, glycosylation, secretion, and biological activity from akikopavolka.com by: Control of human breast cancer by estrogen, growth factors, and oncogenes.

Zhan X, Goldfarb M: Growth factor requirements of oncogene-transformed NIH 3T3 and Balb/c 3T3 cells cultured in defined media. Human transforming growth factor a precursor sequence gene structure, and heterologous expression. In: Cancer Cells 3: Growth factors Cited by: